Original Research

IJMDC. 2017; 1(3): 99-106

A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers

Authors: Soad Nady, Asmaa Ezz, Mohammad A. Mohey, Gamal Esmat, Wafaa Elakel, Mohammad T. Shata.

ABSTRACT

Background: Fibrosis is a major cause of morbidity and mortality worldwide. Biopsy is an invasive procedure. For the evaluation of liver fibrosis, an alternative, noninvasive, new, easy, and available method is determined to assess hepatic fibrosis in Egyptian patients. Methodology: We enrolled 143 patients with fibrosis and they were divided into two groups, severe fibrosis and mild fibrosis group. Th17 cells were stimulated in vitro with S. mansoni soluble egg antigen (SEA), then interleukin (IL)-17, IL-22, and interferon (IFN)-γ were assessed in the culture supernatant. Serum levels of alanine aminotransferase, aspartate aminotransferase, YKL-40, and hyaluronic acid (HA) were also assessed using enzyme-linked immunosorbent assay. Analysis of individual data was conducted to characterize the diagnostic accuracy of the three highly significant fibrosis biomarkers (HA, IL-22, and IFN-γ). Results: A highly significant difference in IL-22, IFN-γ, and HA levels between mild and severe groups was observed. Linear combination of the three biomarkers was selected by the multivariate discriminate analysis as the best combination for construction of the fibrosis discrimination equation. Application of the equation on all patients correctly classified 28.7% with severe fibrosis and 71.3% with mild fibrosis at a discriminant cut-off score (0.297) with 87.18% sensitivity and 85.57% specificity. Conclusion: A simple fibrosis score = [0.594 (numerical constant) + 0.007 HA (ng/ml) + 0.006 IL-22 (ng/ml) − 0.129 IFN-γ (pg/ml)] may be useful for discrimination of severe from mild fibrosis patients without the need of liver biopsy.

Keywords:
liver fibrosis, Th17 cells, sera fibrosis biomarkers


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A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers


Authors
Soad Nady
Zoology Department, Faculty of Science, Helwan University
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Asmaa Ezz
Zoology Department, Faculty of Science, Helwan University
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Mohammad A Mohey
Department of Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University
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Gamal Esmat
Department of Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University
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Wafaa Elakel
Department of Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University
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Mohammad T Shata
Division of Digestive Diseases, Department of Internal Medicine, University of Cincinnati, OH, USA
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Correspondence to:
Soad Nady. Soad Nady, Zoology Department, Faculty of Science, Helwan University; soadnady@science.helwan.edu.eg

Publication history
Received 15 Feb 2018
Accepted 25 Feb 2018
Published in print 08 Mar 2018

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Pubmed Style

Nady S, Ezz A, Mohey MA, Esmat G, Elakel W, Shata MT. A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers. IJMDC. 2017; 1(3): 99-106. doi:10.24911/IJMDC.2.1.5


Web Style

Nady S, Ezz A, Mohey MA, Esmat G, Elakel W, Shata MT. A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers. http://www.ijmdc.com/?mno=291229 [Access: September 25, 2018]. doi:10.24911/IJMDC.2.1.5


AMA (American Medical Association) Style

Nady S, Ezz A, Mohey MA, Esmat G, Elakel W, Shata MT. A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers. IJMDC. 2017; 1(3): 99-106. doi:10.24911/IJMDC.2.1.5


Vancouver/ICMJE Style

Nady S, Ezz A, Mohey MA, Esmat G, Elakel W, Shata MT. A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers. IJMDC. (2017), [cited September 25, 2018]; 1(3): 99-106. doi:10.24911/IJMDC.2.1.5


Harvard Style

Nady, S., Ezz, A., Mohey, M. A., Esmat, G., Elakel, W. & Shata, M. T. (2017) A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers. IJMDC, 1 (3), 99-106. doi:10.24911/IJMDC.2.1.5


Turabian Style

Nady, Soad, Asmaa Ezz, Mohammad A. Mohey, Gamal Esmat, Wafaa Elakel, and Mohammad T. Shata. 2017. A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers. International Journal of Medicine in Developing Countries, 1 (3), 99-106. doi:10.24911/IJMDC.2.1.5


Chicago Style

Nady, Soad, Asmaa Ezz, Mohammad A. Mohey, Gamal Esmat, Wafaa Elakel, and Mohammad T. Shata. "A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers." International Journal of Medicine in Developing Countries 1 (2017), 99-106. doi:10.24911/IJMDC.2.1.5


MLA (The Modern Language Association) Style

Nady, Soad, Asmaa Ezz, Mohammad A. Mohey, Gamal Esmat, Wafaa Elakel, and Mohammad T. Shata. "A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers." International Journal of Medicine in Developing Countries 1.3 (2017), 99-106. Print. doi:10.24911/IJMDC.2.1.5


APA (American Psychological Association) Style

Nady, S., Ezz, A., Mohey, M. A., Esmat, G., Elakel, W. & Shata, M. T. (2017) A novel score for diagnosis of liver fibrosis based on Th17 activity and sera fibrosis biomarkers. International Journal of Medicine in Developing Countries, 1 (3), 99-106. doi:10.24911/IJMDC.2.1.5


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