Original Research Open Access


The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro

Soad Nady, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, Asmaa Ezz.

Abstract
Background: Pollen and pollen products have a long history of use in traditional herbal medicine and have been reported to display a wide range of antimicrobial, antioxidative, anti-inflammatory, antioxidant, and hepato-protective activities. This study aimed to investigate the role of date palm pollen (DPP) ethanolic extract on the formation of granuloma induced by Schistosoma mansoni soluble egg antigen (SEA) in vitro.
Methodology: Peripheral blood mononuclear cells (PBMCs) were isolated from 20 S. mansoni monoinfected, 50 Hepatitis C virus/S. mansoni coinfected patients and 25 healthy individuals. The isolated PBMCs were used for in vitro granuloma induction using SEA conjugated polyacrylamide beads. Different concentrations of DPP ethanolic extract (10 mg/mL, 20 mg/mL, 30 mg/mL, and 40 mg/mL) were added to the granuloma at the first day, then the sizes of granulomas were measured by granuloma index (GI) that was assessed by quantitation of cellular reactivity around the beads after 3, 7, and 14 days.
Results: Treatment of granuloma induced by SEA conjugated polyacrylamide beads with the optimum concen-tration of DPP ethanolic extract (30 mg/mL) resulted in significant (P < 0.05) reduction of GI using PBMCs iso¬lated from Schistosoma monoinfected, hepatitis C virus/Schistosoma coinfected patients or healthy individuals at all the experimental time intervals.
Conclusion: DPP suppressed the effect of SEA and prevented granuloma formation. The results of the present study suggest that DPP could be considered a promising source of new natural anti-inflammatory herbal med¬icine against the S. mansoni associated granulomatous inflammation.

Key words: Date palm pollen, Schistosoma mansoni, granuloma, soluble egg antigen.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Soad Nady
Articles by Irene S Gmail
Articles by Fatma Elzahraa H Salem
Articles by Rabab Salama
Articles by Asmaa Ezz
on Google
on Google Scholar
Article Statistics
 Viewed: 205
Downloaded: 51
Cited: 0

How to Cite this Article
Pubmed Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC. 2017; 1(2): 52-60. doi:10.24911/IJMDC.1.2.3


Web Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. http://www.ijmdc.com/?mno=287573 [Access: February 22, 2018]. doi:10.24911/IJMDC.1.2.3


AMA (American Medical Association) Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC. 2017; 1(2): 52-60. doi:10.24911/IJMDC.1.2.3



Vancouver/ICMJE Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC. (2017), [cited February 22, 2018]; 1(2): 52-60. doi:10.24911/IJMDC.1.2.3



Harvard Style

Nady, S., Gmail, I. S., Salem, F. E. H., Salama, R. & Ezz, A. (2017) The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC, 1 (2), 52-60. doi:10.24911/IJMDC.1.2.3



Turabian Style

Nady, Soad, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, and Asmaa Ezz. 2017. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. International Journal of Medicine in Developing Countries, 1 (2), 52-60. doi:10.24911/IJMDC.1.2.3



Chicago Style

Nady, Soad, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, and Asmaa Ezz. "The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro." International Journal of Medicine in Developing Countries 1 (2017), 52-60. doi:10.24911/IJMDC.1.2.3



MLA (The Modern Language Association) Style

Nady, Soad, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, and Asmaa Ezz. "The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro." International Journal of Medicine in Developing Countries 1.2 (2017), 52-60. Print. doi:10.24911/IJMDC.1.2.3



APA (American Psychological Association) Style

Nady, S., Gmail, I. S., Salem, F. E. H., Salama, R. & Ezz, A. (2017) The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. International Journal of Medicine in Developing Countries, 1 (2), 52-60. doi:10.24911/IJMDC.1.2.3