Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management

Maram Murdhi Alanazi; Rana Alamri; Mona Alshammari; Serien Alharbi; Fatimah Fadhel Alshammari; Yazan O. Arif; Mohammad Al Mohainin

doi:10.24911/IJMDC.51-1765838692

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Online Published: 16 Jan 2026

IJMDC. 2026; 10(1): 485-494

doi: 10.24911/IJMDC.51-1765838692

Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management

Maram Murdhi Alanazi, Rana Alamri, Mona Saad Alshammari, Serien Alharbi, Fatimah Fadhel Alshammari, Yazan O. Arif, Mohammad Al Mohaini.

Abstract
Irritable bowel syndrome (IBS) is a common gastrointestinal disorder characterized by dysbiosis and impaired Short-chain fatty acids (SCFAs) production, but traditionally, dietary fiber interventions lack precision in gut microbiota modulation. Herein, a concept for a new system was introduced: bioengineered strain-specific synbiotics that combine CRISPR-Cas9-modified Bifidobacterium longum strains with metabolically tailored prebiotics aimed at optimal soluble fiber fermentation for targeted IBS management. The probiotics were metabolically engineered to overexpress enzymes involved in butyrate synthesis, while prebiotics were chemically tailored so as not to undergo off-target fermentation, ensuring their preferential use by the bioengineered strains. A quorum-sensing feedback loop was used to dynamically adjust metabolic activity in response to microbial density, thereby sustaining SCFA production along the colon. Moreover, real-time microbiome monitoring via a metagenomics sequencing subsystem self-adjusted the synbiotic dosage to minimize symptom severity while preserving microbial diversity. The closed-loop architecture of this system dynamically phases out plain fiber supplementation when butyrate production reached therapeutic thresholds, thus enabling personalized modulation of gut ecology. Proof of concept testing in vitro achieved a 40% reduction in off-target fermentation of acetylated xylo-oligosaccharides compared to that of native substrates. The herein proposed approach has distinguished advantages over one-size-fits-all static treatments by providing active, precision control over microbial metabolism; traditional therapies have mostly proved ineffective. This study represented one step further in synbiotic design development, capable of transforming the current paradigm of IBS treatment using targeted microbiome modulation.

Key words: Irritable bowel syndrome, synbiotics, gut microbiota, soluble fiber, short-chain fatty acids, fermentation, systematic review.

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Pubmed Style

Alanazi MM, Alamri R, Alshammari MS, Alharbi S, Alshammari FF, Arif YO, Mohaini MA. Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management. IJMDC. 2026; 10(1): 485-494. doi:10.24911/IJMDC.51-1765838692

Web Style

Alanazi MM, Alamri R, Alshammari MS, Alharbi S, Alshammari FF, Arif YO, Mohaini MA. Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management. https://www.ijmdc.com/?mno=303365 [Access: January 25, 2026]. doi:10.24911/IJMDC.51-1765838692

AMA (American Medical Association) Style

Alanazi MM, Alamri R, Alshammari MS, Alharbi S, Alshammari FF, Arif YO, Mohaini MA. Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management. IJMDC. 2026; 10(1): 485-494. doi:10.24911/IJMDC.51-1765838692

Vancouver/ICMJE Style

Alanazi MM, Alamri R, Alshammari MS, Alharbi S, Alshammari FF, Arif YO, Mohaini MA. Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management. IJMDC. (2026), [cited January 25, 2026]; 10(1): 485-494. doi:10.24911/IJMDC.51-1765838692

Harvard Style

Alanazi, M. M., Alamri, . R., Alshammari, . M. S., Alharbi, . S., Alshammari, . F. F., Arif, . Y. O. & Mohaini, . M. A. (2026) Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management. IJMDC, 10 (1), 485-494. doi:10.24911/IJMDC.51-1765838692

Turabian Style

Alanazi, Maram Murdhi, Rana Alamri, Mona Saad Alshammari, Serien Alharbi, Fatimah Fadhel Alshammari, Yazan O. Arif, and Mohammad Al Mohaini. 2026. Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management. International Journal of Medicine in Developing Countries, 10 (1), 485-494. doi:10.24911/IJMDC.51-1765838692

Chicago Style

Alanazi, Maram Murdhi, Rana Alamri, Mona Saad Alshammari, Serien Alharbi, Fatimah Fadhel Alshammari, Yazan O. Arif, and Mohammad Al Mohaini. "Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management." International Journal of Medicine in Developing Countries 10 (2026), 485-494. doi:10.24911/IJMDC.51-1765838692

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Alanazi, M. M., Alamri, . R., Alshammari, . M. S., Alharbi, . S., Alshammari, . F. F., Arif, . Y. O. & Mohaini, . M. A. (2026) Engineered strain-specific synbiotics for precision modulation of soluble fiber fermentation in irritable bowel syndrome management. International Journal of Medicine in Developing Countries, 10 (1), 485-494. doi:10.24911/IJMDC.51-1765838692

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